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Mathias Wind & Wolf D. Lehmann answers a
few questions about this month's fast breaking paper in the field of
Engineering.
From
•>>August 2005
Field:
Engineering
Article Title: Element and molecular mass spectrometry - an emerging analytical dream team in the life sciences
Authors: Wind,
M;Lehmann, WD
Journal: J ANAL ATOM SPECTROM
Volume: 19
Page: 20-25
Year: JAN 2004
* German Canc Res Ctr, DKFZ, Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
* German Canc Res Ctr, DKFZ, D-69120 Heidelberg, Germany.
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 Why
do you think your paper is highly cited?
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“The article describes the benefits that can be achieved when two analytical techniques which are normally strictly separated are jointly applied to an analytical problem in the life sciences.”
~Prof. Dr. Wolf D. Lehmann
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This review describes recent work on the combined use of
element and molecular mass spectrometry (ESI-MS or MALDI-MS) in
the life sciences. The review has a very narrow focus on those
studies, where the combination of both techniques could be
channeled into a final high-quality result, which would not have
been possible to achieve by either of these techniques alone.
Does
it describe a new discovery or a new methodology that's useful to
others?
The article describes the benefits that can be achieved when
two analytical techniques which are normally strictly separated
are jointly applied to an analytical problem in the life
sciences. Element mass spectrometry is a typical method in
inorganic analysis, whereas molecular mass spectrometry is
typically applied to organic samples. Reading about the examples
of a successful combination of these techniques—normally only
one of these techniques is applied—the reader may be inspired
to find new or more elegant solutions for analytical problems in
his or her own area of research.
Could
you summarize the significance of your paper in layman's terms?
The phenomenon of life does not care about the separation
between organic and inorganic matter, it uses metals as well as
macromolecules composed of carbon, oxygen, etc. This review
substantiates that the integration of inorganic and organic
microanalysis is a way to achieve more meaningful results in the
bioanalytical sciences.
How
did you become involved in this research?
We were in need of a robust and quantitative methodology for
detection of phosphopeptides and phosphoproteins. We
successfully tested and then introduced element mass
spectrometry with phosphorus detection for this purpose. See
[1,2] below.
References:
[1] Wind, M.; Edler, M.; Jakubowski, N.; Linscheid, M.; Wesch,
H.; Lehmann, W. D. "Analysis
of protein phosphorylation by capillary liquid chromatography
coupled to element mass spectrometry with 31P detection and to
electrospray mass spectrometry," Anal. Chem. 73:
29-35, 2001.
[2] Wind, M.;
Feldmann, I.; Jakubowski, N.; Lehmann, W. D. "Spotting and
Quantification of Phosphoproteins purified by gel
electrophoresis by laser ablation element mass spectrometry with
phosphorus-31 detection," Electrophoresis 24,
1276-1280, 2003.
Prof. Dr. Wolf D. Lehmann
Central Spectroscopy
German Cancer Research Center (DKFZ)
Heidelberg, Germany
Mathias Wind
Analytics
Basilea Pharmaceutica
Basel, Switzerland
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ESI Special Topics,
August 2005
Citing URL - http://www.esi-topics.com/fbp/2005/august05-Wind_Lehmann.html
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